Figure 1.

Fusaric acid induced global DNA hypomethylation in HepG2 cells. DNA isolated from control and FA-treated HepG2 cells were assayed for global DNA methylation by quantifying 5-methylcytosine using a Colorimetric Methylated DNA Quantification Kit. Fusaric acid decreased the percentage of 5-methylcytosine in HepG2 cells compared to the control. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (**p < 0.005, ***p < 0.0001).