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. 2019 May 16;14(8):804–817. doi: 10.1080/15592294.2019.1615358

Figure 5.

Figure 5.

The effect of FA on the ubiquitination of DNMT1, DNMT3A, and DNMT3B in HepG2 cells. (a) The ubiquitination of DNMT1, DNMT3A, and DNMT3B were detected by immuno-precipitation and Western blot. Fusaric acid altered the ubiquitination of DNMT1, DNMT3A, and DNMT3B in HepG2 cells. (b) RNA isolated from control and FA-treated HepG2 cells were reverse transcribed into cDNA and analyzed by qPCR. Fusaric acid significantly decreased the expression of UHRF1 and USP7 in HepG2 cells. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (*p < 0.05, **p < 0.005, ***p < 0.0001).