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Effect of DNAzyme split mode on catalytic activity. (A) Constructing the 1:3 mode split DNAzyme and subsequent conjugation to COC-5334 via dinucleotide linkers to generate CBSAzyme-5334–13. Structures and time-course absorbance measurements for (B) CBSAzyme-5334–13 and (C) CBSAzyme-5334–22 in the absence and presence of 250 μM cocaine. [Each fragment] = 1 μM.
