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. 2019 Jul 9;17(7):e3000332. doi: 10.1371/journal.pbio.3000332

Fig 1. The seminal content and olfactory potency of spermine.

Fig 1

(A) The concentration of spermine in sea lamprey milt and ovarian fluid with eggs (mean ± SEM, n = 6), measured with UHPLC–MS/MS. (Inset) Structure of spermine. (B) Percentage of spermine by weight in the milt components, seminal plasma and sperm, determined with UHPLC–MS/MS (mean ± SEM, n = 6). (C) Semi-logarithmic plot of normalized EOG amplitude (mean ± SEM) elicited by spermine (10−15 M to 10−6 M) in pre-ovulatory female (n = 10) and pre-spermiating male (n = 11) sea lampreys. The EOG response to spermine at each concentration was blank-subtracted and normalized to the response of 10−5 M L-ARG (standard) for each fish. (D) Representative EOG traces of pre-ovulatory female olfactory epithelia exposed to spermine at concentrations between 10−15 and 10−6 M. The number above each trace is the logarithmic value of the molar concentration of each stimulant. The bar above the L-ARG trace on the left represents the duration of odorant treatment. (E) EOG traces of pre-spermiating male olfactory epithelia exposed to spermine. Underlying data are available in S1 Data. blank, vehicle solution; EOG, electro-olfactogram; L-ARG, L-arginine; N.D., not detectable; UHPLC–MS/MS, ultrahigh performance liquid chromatography-tandem mass spectrometry.