Fig 1. Fes1 is required for degradation of Fbp1 by Vid.

Panels A-C show immunoblots probing for Fbp1. (A) Cells starved of glucose for three days were transferred to glucose-rich medium, which triggers Vid, and Fbp1 abundance was monitored by western analysis for three hours. Strains are 1075 (wt), SY136 (center) or SY135 (right). SY136 and SY135 express Ssa2 or Ssa1, respectively, as the only Ssa Hsp70 in the cell. Image labeled "load" shows the blotted membrane stained by amido-black as loading and transfer controls. (B) As in panel (A) using strains SY346, SY351 and BY241ΔSSE, which lack NEFs Fes1, Snl1 or Sse1 as indicated. (C) As in panel (A) except glucose was restored after starving cells for one day, which triggers degradation of Fbp1 by proteasomes. (D, E) Plots of data represented by experiments in panels (A-C) quantified using Image-J from at least 3 independent replicates of strains starved for three days (D) or one day (E). Error bars represent SD.