Table 4. Plasmids used in this study.

Plasmid	Description	Source
pRS425	2μ, LEU2 (multi-copy)	[55]
p415	CEN, LEU2 (single-copy)	[30]
pRS314	CEN, TRP1 (single-copy)	[54]
pRS315	CEN, LEU2 (single-copy)	[54]
p315FES1	pRS315, PFES1::FES1	This study
p315FES1RA	pRS315, PFES1::fes1A79R,R195A	This study
p315FES1ΔRD	pRS315, PFES1::fes1Δ2–34	This study
p315FES1-GST	pRS315, PFES1::FES1-GST	This study
p315FES1RA-GST	pRS315, PFES1:: fes1A79R,R195A-GST	This study
p315FES1ΔRD-GST	pRS315, PFES1::fes1ΔRD-GST	This study
p313-GST	pRS313, PFES1::GST	This study
p415ADH	p415, PADH1	This study
p415ADHFES1	p415, PADH1::FES1	This study
p415ADHFES1RA	p415, PADH1::fes1A79R,R195A	This study
pET28aFBP1	E. coli expression, N-His6 tag	This study
pET28aFBP1P2A	E. coli expression, N-His 6tag	This study
pET28aFBP1P2Δ	E. coli expression, N-His6 tag	This study
pET28aFBP1-P2AS12AT13A	E. coli expression, N-His6 tag	This study
pET28aFBPΔ2–12	E. coli expression, N-His6 tag	This study
pET24-SSA1	E. coli expression, No tag	[51]
pMR299 (Ssa2)	E. coli expression, No tag	[52]
pGEX5AFES1	E. coli expression, N-GST tag	[15]
pGEX5AFES1RA	E. coli expression, N-GST tag	This study
pGEX5AFES1ΔRD	E. coli expression, N-GST tag	This study
pRS425SSE1	SSE1 wild type	[37]
pRS425SSE1K69Q	sse1K69Q mutant	[37]
pRS425SSE1G233D	sse1G233D mutant	[37]