Fig 4. Relationship between estimated fitness effects of mutations obtained by MCMC and estimates obtained from the difference in mean growth rate between recombinant lines carrying the mutant and wild-type allele (raw difference).

Raw difference estimates were calculated within MA line genotypes, excluding the ancestral lines (which are homozygous mutant or wild type for all mutations carried by a MA line). MA, mutation accumulation; MCMC, Markov chain Monte Carlo.