Figure 3.
Antroalbol H does not affect Akt. A–D, immunoblots of p-AktS473, p-AktT308, t-Akt, and β-actin in cells stimulated by AH for a short time. L6 myotubes or 3T3-L1 adipocytes were incubated with 100 nm insulin or 10 μm AH for 15 min or 4 h. E and F, the same assay was performed in L6 myotubes or 3T3-L1 adipocytes when cells were incubated with 100 nm insulin or 2.5–20 μm AH for 24 h. The band intensity of the phosphorylation protein was normalized to that of total protein in the right panel (the same as below). The values are expressed as -fold change relative to the value from the DMSO-treated cells (Con). Results are represented as means ± S.E. (error bars) (n = 3). **, p < 0.01 compared with control.