Figure 10.

PKD and Gβγ-dependent mechanical hyperalgesia. Mice were pretreated with CRT0066101 (80 mg/kg, orally), gallein (100 mg/kg, intraperitoneally), or vehicle (orally or intraperitoneally) for 2 h (CRT0066101) or 30 min (gallein) before intraplantar injection of trypsin (140 nm) (A, E, D, and H), CS (2.5 μm) (B and F), or NE (1.18 μm) (C and G) (all 10 μl). A–C and E–G, VFF withdrawal responses of the ipsilateral protease-injected paws. D and H, VFF withdrawal responses of the contralateral noninjected paws. Numbers in parentheses indicate mouse numbers. *, p < 0.05; **, p > 0.01; ***, p > 0.001; ****, p < 0.0001 (two-way ANOVA, Bonferroni multiple comparisons). Error bars, S.E.