. 2019 May 24;294(27):10553–10563. doi: 10.1074/jbc.RA119.008610

Table 3.

Comparison of the measured rate constants of folding in 60 mm GdnHCl of FimA monomers and the FimAa constructs with the rate constants predicted from the Chevron plots in Fig. 4B

FimA construct	Measured k_F in 60 mm GdnHCl^a (s⁻¹)	Calculated k_F in 60 mm GdnHCl^b (s⁻¹)	(k_calc^F)/(k_measured^F)	(k_F(FimA))/(k_F(FimAa))
FimA^ECO	9.50 ± 0.08 × 10⁻⁵	NA^c		1.03
FimAa^ECO	9.26 ± 0.09 × 10⁻⁵	1.09 ± 0.28 × 10⁻⁴	1.18	1.03
FimA^SHI	2.08 ± 0.01 × 10⁻⁴	NA		1.32
FimAa^SHI	1.57 ± 0.01 × 10⁻⁴	1.81 ± 0.28 × 10⁻⁴	1.15	1.32
FimA^SAL	1.05 ± 0.01 × 10⁻³	NA		0.97
FimAa^SAL	1.08 ± 0.02 × 10⁻³	7.42 ± 1.09 × 10⁻⁴	0.69	0.97

^a Rate constants of folding at pH 7.0 and 25 °C in 60 mm GdnHCl, determined from the refolding experiments in Fig. 5.

^b Rate constants of folding at pH 7.0 and 25 °C in 60 mm GdnHCl, calculated from the Chevron plots in Fig. 4B and the deduced values of k_F^H2O and m_F in Table 2.

^c NA, not applicable.