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. 2019 May 22;294(27):10438–10448. doi: 10.1074/jbc.RA119.007755

Figure 7.

Figure 7.

Overexpression of miR-223 rescues Min6 β-cell by improving proliferation response and viability. A, representative images showed Ad.GFP- or Ad.miR-223–infected Min6 β-cells. B, expression of miR-223 mRNA was measured by qPCR. C and D, proliferation of Min6 cells was significantly increased upon up-regulation of miR-223 as shown by representative FACS scatter plots (C) and quantification (D) of Ki-67–positive cells. E, MTS assay showed that Min6 cell viability was increased after miR-223 overexpression followed by 24 h palmitate (0.5 mm) or 1 h H2O2 (200 mm) treatments. F, protein levels of Foxo1, Sox6, and p27 were decreased, whereas p-Foxo1, Pdx1 and Glut2 levels were increased 48 h after Ad.miR223 infection. H, key genes related to β-cell identity and proliferation were measured after overexpressing miR-223. I, glucose-stimulated insulin secretion assay showed that miR-223 down-regulation impaired insulin secretion, which could be rescued by overexpression of miR-223. Data are shown as mean ± S.E. (error bar).*, p < 0.05; **, p < 0.01; ***, p < 0.001 versus controls by t test.