Figure 2.

Ligation experiments in the presence of XRCC4–ligase IV and Ku 70/80. A, comparative functional properties of TdT WT, Pol μ, and TdT-μ chimera using complementary or noncomplementary overhangs. B, sequences of the products of ligation for compatible overhangs. Note that only the top strand of sequence is shown. The junctions are where the Additions column is located, and when no nucleotides are present here, this means that the left and right ends are joined directly, using the compatible overhangs. C, sequences of the products of ligation for incompatible overhangs. Note that only the top strand of sequence is shown.