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Targeted and semitargeted mutations induced in the progeny from the 5fC-linked DpC construct in HEK 293T (293T) and various polymerase KO or knockdown cells (as indicated). The data represent the mean and standard deviation (of the total MF) from two to five independent experiments. The statistical significance of the difference in MFs between HEK 293T and TLS Pol knockouts and/or knockdowns (KD) was calculated using two-tailed, unpaired Student's t test (*, p < 0.05; **, p < 0.005).
