Western blot analysis.
Notes: (A) Simplified KIT-signaling pathway. Immunoblot evaluation of KIT, phospho-KIT, PTEN, AKT, phospho-AKT, mTOR, phospho-mTOR, MAPK, and phospho-MAPK in GIST48 (B) and GIST48B (C). S, BYL719-sensitive; R, BYL719-resistant.