Table 1.

GIST cell-lines ^a characteristics

	Origin	KIT mutations and other characteristics	Growth mediumb
GIST48	GIST primary/patientc	Primary, homozygous KIT exon 11 (V560D) mutation; secondary, heterozygous KIT exon 17 (D820Ad) mutation.	IMDM + 15% FBS
GIST48B	Subline of GIST48	Retains primary mutation (KIT V560D) in all cells; nearly undetectable KIT transcript and protein; secondary, heterozygous KIT exon 17 (D820Ad) mutation; keeps downstream KIT signaling active	IMDM + 15% FBS

Notes: ^aKindly provided by Dr Fletcher (Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA); ^breagents purchased from Thermo Fisher Scientific (Waltham, MA); ^cestablished from a GIST patient that had progressed after initial response to imatinib therapy; ^dlocated in the kinase-activation loop, confers resistance to imatinib and sunitinib.

Abbreviations: IMDM, Iscove's Modified Dulbecco's Medium; FBS, Fetal Bovine Serum.