TABLE I.

Assessment of Student Learning

Program learning outcome		Specific outcome objectives	Assessment instrument	Defines success level	Outcomes for the first year of this laboratory exercise	Refinement for future
1.	Students will acquire laboratory skills necessary to conduct an experiment in CRISPR gene editing using yeast as a model Experimental system.	Students will demonstrate ability to perform technical tasks and analyze results from a CRISPR experimental protocol.	Assessment will be through a lab reporta that summarizes the students’ research experience in the CRISPR lab.	At least 70%of class earns 60% of available points for CRISPR lab report. Grades are not dependent on expected results, but understanding of concepts.	The average grade for this lab report, over 7 laboratory sections, with a total of 152b students, was 8.5%. More than 90% students achieved a grade of 80% or better.
2.	Students will develop an understanding of the biological principles underlying CRISPR gene editing technology.	Students will be able to explain the biology that enables the CRISPR gene editing process, and understand the biological basis for each experimental step used	This outcome will be addressed through laboratory quizzes and an exam. given during recitation, that covers material relevant to the CRISPR lab protocol	At least 70%of class earns 60% of available points for this exam	We did not reach the 70% goal for this teaching outcome. Questions and feedbackc from students indicated that some students had difficulties with the extensive terminology associated with the CRISPR lab protocol.	We will emphasize the terminology more, in particular emphasizing the difference between guide RNAs and homology directed repair fragments. This emphasis will be incorporated into a recitation lecture, and reviewed at the beginning of the CRISPR lab session by the teaching assistants.
Future goal
3.	Students will develop an appreciation for the biological significance of CRISPR gene editing techlology	Learn the basics of how scientists use CRISPR gene editing technology to make specific genome modifications in experimental organisms, and how this technology can be used for medical, commercial, and agricultural applications.	Students will be asked to include an example of a societal use of CRISPR technology. This will be an independent project, and will be included as a short paragraph in their lab reporta.	At least 70%of class earns 70% of available points for this supplemental portion of their lab report.

^aA detailed rubric of assessments through lab reports is included in Table 2.

^bFourteen students resigned while the laboratory exercise was in process.

^cRepresentative comments from students are included in the main text. A student evaluation questionnaire can be found in supporting information 5.