Video 1. Single cells are precisely illuminated by ATIM via in situ analysis and tracking of bacteria.

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The left panel shows the merged images of gfpASV and mCherry fluorescence microscopy images over time. The right panel shows the merged images of red LED projected patterns and bright field images corresponding to the left panel. The fluorescence intensity of gfpASV in the illuminated cells and their offspring (colored red in right panel) is significantly increased after using ATI for 460 mins. In contrast, the gfpASV fluorescence intensity of the un-illuminated cells remains low and these cells remain motile.