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. 2019 Jun 25;55(2):451–461. doi: 10.3892/ijo.2019.4831

Figure 7.

Figure 7

Overexpression of sorcin partially recovered the effect of miR-1 on cell apoptosis and drug accumulation. (A) The cells apoptosis rates of SGC7901/ADM and SGC7901/VCR cells following transfection of miR-NC, miR-1 mimics and miR-1 mimics with pcDNA-sorcin followed by the treatment of ADM and VCR, respectively. The apoptosis rates were measured by flow cytometric analysis. (B) The cell apoptosis associated proteins, including c-fos, c-jun, BCL-2 and Bax were analyzed in SGC7901/ADM and SGC7901/VCR cells following transfection of miR-NC, miR-1 mimics and miR-1 mimics with pcDNA-sorcin. (C) The drug accumulation of Rhodamine 123 in SGC7901/ADM and SGC7901/VCR cells following transfection of miR-NC, miR-1 mimics and miR-1 mimics with pcDNA-sorcin were analyzed by flow cytometry. (D) The expression levels of MDR1/P-gp and MRP-1 were analyzed in SGC7901/ADM and SGC7901/VCR cells following transfection of miR-NC, miR-1 mimics and miR-1mimics with pcDNA-sorcin. Data are presented as the mean ± standard deviation of three repeated experiments. *P<0.05, **P<0.01. miR, microRNA; VCR, vincristine; ADM, adriamycin; miR-NC, scramble control; BCL-2, B cell lymphoma-2; Bax, Bcl-2-associated X protein; MDR1/P-gp, multidrug resistance protein 1/P-glycoprotein; MRP-1, multidrug resistance-associated protein 1.