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. 2019 Jul 2;55(2):347–358. doi: 10.3892/ijo.2019.4835

Figure 2.

Figure 2

Primary AML cells can be infected with MeV. The PBMC fraction of patients with AML were infected with MeV-GFP or MOCK infected and analyzed at 4 dpi. (A) GFP expression of PBMCs from UPN 1 or UPN 2 was investigated by FACS. The percentage of positive cells is indicated. One representative result out of 3 experiments with similar results is shown. (B) Cell number was determined using the Countess Automated Cell Counter. The percentage of living cells is indicated. UPN 1 (n=5); UPN 2 (n=2). (C) Viability of cells was measured using CellTiter-Blue viability assay. UPN 1 (n=5); UPN 2 (n=1). (D) Apoptosis was determined by staining with TMRE and subsequent FACS analysis. The percentage of TMRE negative cells is indicated. UPN 1 (n=3); UPN 2 (n=1). (B-D) Values depict means of data from the indicated number of independent experiments with standard deviation. Statistically significant differences (P<0.05) are indicated by asterisk (*); ns, not significant. AML, acute myeloid leukemia; MeV, measles vaccine virus; TMRE, tetramethylrhodamine ethyl ester; UPN, unique patient number; PBMCs, peripheral blood mononuclear cells.