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. 2019 May 30;125(2):170–183. doi: 10.1161/CIRCRESAHA.118.314515

Figure 1.

Figure 1.

SRSF3 (serine/arginine splicing factor 3) expression in the heart decreases with age. A, In situ hybridization for SRSF3 performed at different embryonic stages, either in whole mount (Left set: E9.5, E10.5, and E11.5) or 10 μm sections (Right set: E12.5, E13.5, and E14.5). The region including the developing heart is shown at high magnification to the right of each whole-embryo view. B, qRT-PCR (polymerase chain reaction) analysis of SRSF3 mRNA expression (exons 3–5) normalized to Gapdh expression in hearts extracted from C57BL/6 mice at different time points. Data are shown as mean±SEM; n=3–4 mice per group. **P<0.01, ***P<0.001 vs E12.5, 1-way ANOVA followed by the Bonferroni multiple comparison test. C, Immunofluorescence analysis of hearts isolated from C57BL/6 mice at different ages using anti-SRSF3 (red), anti-TroponinT (green), and DAPI (blue). M, month; Bars, A, 1 mm (whole mount, full embryo); 0.5 mm (whole mount, heart); 2 mm (embryo section), 0.5 mm (heart section); C, 50 μm.