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. 2019 May 30;125(2):170–183. doi: 10.1161/CIRCRESAHA.118.314515

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© 2019 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 2. — SRSF3 (serine/arginine splicing factor 3) is downregulated after myocardial infarction (MI) by alternative splicing. A, Schematic of the SRSF3 mRNA isoforms. The yellow box represents alternative exon 4. B and C, Analysis by qRT-PCR (polymerase chain reaction; B) and Western blot (C) of SRSF3 mRNA (exons 3–5 normalized to Gapdh) and protein expression in the remote myocardium of mouse hearts 28 days after induction of MI by left coronary artery ligation. MW markers are indicated. D, qRT-PCR analysis of the SRSF3 NMD (nonsense-mediated decay) isoform in the remote myocardium, normalized to total SRSF3 mRNA expression. E, Western blot analysis of SRSF1 protein expression in the remote myocardium. Data in B and D are mean±SEM, and symbols represent individual animals (n=7–8); *P<0.05 and ***P<0.001, Student t test.