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. 2019 May 30;125(2):170–183. doi: 10.1161/CIRCRESAHA.118.314515

Figure 7.

Figure 7.

SRSF3 (serine/arginine splicing factor 3) regulates mTOR (mammalian target of rapamycin) splicing, and its depletion induces decapping of contraction-related mRNAs. A, mTOR long and short isoforms. B, Western blot analysis of mTOR splicing isoforms and the phosphorylation states of AKT and 4E-BP1 in hearts of control and SRSF3 knockout (KO) mice on day 2 post tamoxifen induction. C–F, qRT-PCR (polymerase chain reaction) analysis of SERCA2A (C), RyR2 (D), MYBPC3 (E), and MYH7 (F) decapped mRNAs in hearts of tamoxifen-induced control and SRSF3 KO mice. Data are normalized to total mRNA expression and are shown as mean±SEM; n=5–9 mice per group. **P<0.01 and ***P<0.001 for SRSF3 KO vs Control; ##P<0.01 and ###P<0.001 vs day 1; 2-way ANOVA followed by the Bonferroni post-test. 4E-BP1 indicates eIF4E-binding protein 1; eIF4E, eukaryotic translation initiation factor 4E; MYBPC3, myosin-binding protein C3; MYH7, myosin heavy chain 7; RYR2, ryanodine receptor 2; and SERCA2A, sarcoplasmic/endoplasmic reticulum calcium ATPase 2a.