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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: J Immunol. 2019 Jun 7;203(2):511–519. doi: 10.4049/jimmunol.1900363

FIGURE 3.

FIGURE 3.

Defective generation of cDC1 from CD11cCreWnt4flox/flox BM progenitors cultured with Flt3L plus GM-CSF. (A) Gating strategy to identify cDC from BM progenitors cultured for 12 days in RPMI CM+ 100 ng/ml Flt3L+ 10 ng/ml GM-CSF. (B) Representative flow plots showing CD24+ (cDC1) and CD172a+ (cDC2) in control vs CD11cCreWnt4flox/flox vs. Batf3−/− after gating as in “A”. (C and D) Quantification of cDC populations showing (C) percentage and (D) cell number of each cDC subset as gated in “B”. (E) Expression of CD103 and Clec9A within each cDC population. (F) Frequency and (G) Cell number of cDC subsets as shown in “E”. Representative results from 2 experiments. Graphs show mean ± SEM n=5/group with * p< 0.05 and ** p<0.01 by ANOVA.