CD11c-dependent Wnt4 disrupts tissue cDC homeostasis. (A) Gating strategy to identify cDCs. (B-E) Frequencies (B, D) and cell number (C, E) of cDC populations in spleen (B, C) and small intestine (SI) (D,E). (F- H) (F) Gating strategy, (G) representative flow plots and (H) Quantification of small intestine cDC populations from CD11cCre vs CD11cCre-Wnt4flox/flox based on expression of CD172a and CD103 (DP= CD103 and CD172a double positive). Graphs show Mean ± SEM from N=3–4 biological replicates per condition with * p< 0.05 and *** p< 0.005 as determined by t-test or two-way. Representative results from 3 independent experiments.