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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Mol Neurobiol. 2019 Jan 16;56(8):5568–5585. doi: 10.1007/s12035-019-1467-8

Figure 7. Inhibition of mGluR1 signaling attenuated the microglia phagocytosis of synapses induced by amyloid fibrils.

Figure 7.

JNJ16259685 significantly decreased the internalization of PSD95 with lysosome marker CD68 in microglia (Iba1) in the rats injected with amyloid fibrils (a, n = 5 rats in each group, F3,16 = 73.0, P <0.0001, scale bar = 10 μm). Each dot represents the mean value of 4 brain sections of one animal. Microinjection of JNJ16259685 significantly recovered the amplitude (b, n = 13 rats in each group, Kruskal-Wallis Statistic KW = 18.2, P = 0.0004) and inter-event interval of mEPSCs (b, n = 13 rats in each group, F3,48 = 16.8, P<0.0001) in the hippocampal CA1 neurons in the rats injected with amyloid fibrils. Microinjection of JNJ16259685 also shortened the escape latency (c, n = 10 rats in each group, effect of group [F3,36 = 13.3, P<0.0001], effect of time [F4,36 = 135.5, P<0.0001], interaction between group and time [P = 0.47]) and increased the time spent in the target quadrant (d, n = 10 rats in each group, F4,36 = 6.57, P = 0.0012) in the amyloid-injected rats. (d) Representative path tracings in each quadrant during the probe trial on day 6 (T, target quadrant; R, right quadrant; O, opposite quadrant; L, left quadrant). Data represent mean ± s.e.m. For box-and-whiskers plots, the box extends from the 25th to 75th percentiles, a line within the box marks the median. Whiskers (error bars) above and below the box represent the minimum and maximum values. *P<0.05, **P<0.01, ***P<0.001.