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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: J Immunol. 2019 Jun 5;203(2):370–378. doi: 10.4049/jimmunol.1900388

FIGURE 5.

FIGURE 5.

Helios deficient OT-II cells differentiate into pTregs. (A) Naïve CD4+ T cells from Ikzf2fl/fl × CD4Cre OT-II Foxp3GFP or control mice were adoptively transferred into congenic F1 recipients. Recipient mice were immunized s.c. the following day with OVA/CFA. Draining LNs were harvested on d11 or (B) the indicated days after immunization, gated on CD45.2+ OT-II cells and analyzed for Foxp3 expression. (C) Naïve OT-II T cells were adoptively transferred into congenic F1 recipients. Recipient mice were given OVA in drinking water ad lib. for 7 d. Foxp3 expression in CD45.2+ OT-II cells was measured in mLN and PP. (D) Naïve OT-II cells were adoptively transferred as in (A) and recipient mice were immunized s.c. the following day with OVA/IFA. Foxp3 expression in CD45.2+ OT-II cells was analyzed from the dLN on day 7.