Skip to main content
. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: J Immunol. 2019 Jun 5;203(2):370–378. doi: 10.4049/jimmunol.1900388

FIGURE 7.

FIGURE 7.

The generation of pTreg in Helios deficient cells suppresses restimulation in an antigen specific manner. (A) Experimental outline of OT-II sequential transfer. Naïve CD45.2+ OT-II cells were adoptively transferred into congenic F1 mice (CD45.1 × CD45.2), which were immunized s.c. the next day with OVA/CFA. On d9, CD45.1+ OT-II cells were transferred into recipient mice followed by OVA/IFA immunization on day 10. (B) Draining LN were harvested on day 14 and the proliferation of CD45.2+ and CD45.1+ OT-II cells was analyzed by FACS. (C) Experimental outline of OT-II/ SMARTA sequential transfer. As in (C) CD45.1+ SMARTA cells were transferred on d9 followed by s.c. immunization with OVA + GP66-77 in IFA. (D) Draining LNs were harvested on day 14 and the proliferation of CD45.2+ OT-II cells and CD45.1+ SMARTA cells was analyzed.