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. 2019 Jul 9;8(7):giz081. doi: 10.1093/gigascience/giz081

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© The Author(s) 2019. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4: — The evolutionary pattern and the origin of the top 30 putative toxins. The node numbering refers to the nodes in Fig. 3a. Putative toxins present in Dasypogon diadema but missing in Eutolmus rufibarbis or Machimus arthriticus are coloured red. Single-copy genes: putative toxins with only 1 copy on the protein-coding genome of D. diadema; multi-copy genes*: protein-coding genes that belong to orthogroups assembled of ≥2 protein-coding genes in D. diadema. Only 1 member of the orthogroup is present in the venom; multi-copy genes**: protein-coding genes that belong to orthogroups assembled of ≥2 protein-coding genes in D. diadema. Two or more members of the same orthogroup are present in the venom.