Table 1.
Identified phosphopeptides for purified CrzA protein from multiple genetic backgrounds
| Phosphorylated Residue | Peptide Sequencea | Localization Probabilityb | Ambiguity Score (Ascore)c | Peptide Scored | Mascot Delta Ion Scoree | Total Peptides (WT)f | Total Peptides (ΔcnaA)f | Total Peptides (ΔpkaC1)f |
|---|---|---|---|---|---|---|---|---|
| S282 | I[pS][pS]AAPSPYLSQHE | 100% | 45.42 | 107.87 | 29.21 | 0 | 2 | 0 |
| S283 | I[pS][pS]AAPSPYLSQHE | 100% | 47.48 | 107.87 | 29.21 | 0 | 4 | 0 |
| S287 | ISSAAP[pS]PYLSQHE | 100% | 24.95 | 157.87 | 28.68 | 0 | 5 | 0 |
| S424 | IP[pS]FGPSKPASNLDSLSPPPSSTR | 100% | 94.68 | 318.75 | 73.77 | 2 | 8 | 6 |
| S432 | IPSFGP[pS]KPA[pS]NLD[pS]LSPPPSSTR | 96% | 14.24 | 103.97 | 20.98 | 0 | 1 | 0 |
| S436 | IPSFGP[pS]KPA[pS]NLD[pS]LSPPPSSTR | 99% | 24.95 | 161.18 | 33.75 | 0 | 2 | 0 |
| S438 | IPSFGPSKPASNLDSL[pS]PPPSSTR | 100% | 43.97 | 262.97 | 49.13 | 5 | 6 | 15 |
| S450 | GR[pS]K[pS]DPYAHPSTSR | 100% | 62.38 | 181.38 | 23.36 | 0 | 5 | 0 |
| S452 | GR[pS]K[pS]DPYAHPSTSR | 100% | 69.51 | 181.38 | 23.36 | 5 | 31 | 5 |
| S461 | SK[pS]DPYAHPST[pS]RLR | 97% | 20.2 | 64.31 | 15.31 | 0 | 1 | 0 |
| S467 | SS[pS]TSSSLDPLAPTTPR | 100% | 30.97 | 327.02 | 94.37 | 1 | 1 | 1 |
| S471 | SSSTSS[pS]LDPLAPTTPR | 98% | 24.95 | 191.19 | 29.27 | 0 | 0 | 1 |
| S482 | [pS]L[pS]PFDSFGR | 100% | 145.71 | 143.56 | 38.28 | 12 | 29 | 7 |
| S484 | [pS]L[pS]PFDSFGR | 100% | 122.33 | 78.55 | 19.77 | 15 | 37 | 11 |
| S512 | L[pS]TSSIDSR | 100% | 55.92 | 165.8 | 56.51 | 6 | 15 | 4 |
| T513 | RL[pS][pT]SSIDSR | 100% | 30.97 | 60.07 | 22.62 | 0 | 2 | 0 |
| S515 | RL[pS]T[pS][pS]IDSR | 100% | 20.98 | 105.24 | 31.02 | 0 | 4 | 0 |
| S534 | NYILGLADPQRPGA[pS]PNDSKR | 100% | 83.65 | 132.45 | 23.72 | 2 | 21 | 2 |
| S599 | KRHEGLH[pS]GEK | 100% | 1,000.00 | 17.15 | 13.33 | 0 | 4 | 0 |
| S649 | AGRICIKPLLDEE[pS]QERE | 100% | 1,000.00 | 222.9 | 32.63 | 3 | 5 | 5 |
Sequence of cleaved peptide containing phosphorylated residue of interest with highest localization probability. Predicted phosphorylated residues are shown as [pS/T], and residue of interest is shown in bold.
Percent probability that predicted phosphorylation site of interest is accurately identified as phosphorylated within the peptide indicated. Based on Ascore.
Statistical metric assessing probable localization accuracy for the indicated phosphorylation site. Ascore of >19 indicates >99% probability of accurate site prediction (Beausoleil et al. 2006).
Probability-based ion matching score for indicated phosphorylation site (Beausoleil et al. 2006).
Statistical metric for assigning phosphorylation to specific indicated site using Mascot proteomic analysis software (Matrix Science, Boston, MA).
Total number of peptides identified in the indicated genetic background (WT, ΔcnaA, or ΔpkaC1) via MS analysis demonstrating phosphorylation at the indicated residue with localization probabilities of >95 percent based on Ascores.