Fig. 6.

In part 6A, a DRG neuron is shown after treatment with mNCX-1 siRNA for 4 days after removal of the siRNA (see Figure 1). DRG cultures have been treated with antibodies to type 3 beta tubulin and stained with a secondary body labeled with Alexa dye 488 to display the neuronal structure (green). In part 6B, the same neuron has been co-stained with antibodies to mNCX-1 and a secondary antibody labeled with Alexa dye 555 (red). For a comparative purposes, the same reagents as utilized in siRNA -treated cultures (6A and 6B), control neurons of the same maturity were stained with type 3 beta tubulin in 6 C and in combination with mNCX-1 and type 3 beta tubulin immunofluorescence (green) in 6 D. The calibration bar represents 100 μ.