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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: J Immunol. 2019 Jun 5;203(2):500–510. doi: 10.4049/jimmunol.1900262

Figure 3. Neutrophils release NETs in response to FM-CM.

Figure 3

Neutrophils were exposed to no treatment (NT); unstimulated FM-CM (FM); LPS-stimulated FM-CM (FM+LPS); or LPS alone, after which: (A) Extracellular DNA (green) of released NETs colocalized with citrullinated histone 3 (red) or neutrophil elastase (red). Images are representative of three independent experiments. Scale bar= 100μm. (B) NETs were visualized using Sytox Green (arrowed). Images are representative of five independent experiments. (C) Extracellular DNA was quantified using PicoGreen (n=13). *p<0.05, **p<0.01, ***p<0.001 compared to NT unless otherwise indicated.