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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: J Immunol. 2019 Jun 5;203(2):500–510. doi: 10.4049/jimmunol.1900262

Figure 5. LPS-stimulated FM-CM induce neutrophil ROS production which contributes to downstream IL-8 secretion and NET release.

Figure 5

Neutrophils were exposed to no treatment (NT); unstimulated FM-CM (FM); LPS-stimulated FM-CM (FM+LPS); or LPS alone in the presence of media or the ROS inhibitor, diphenyleneiodonium (DPI). (A) Barchart shows ROS production by neutrophils under media conditions in relative light units (RLU, n=6). (B-D) In the presence of either media or DPI (n=7), barcharts show: (B) IL-8 secretion; (C) MPO degranulation; and (D) NET-associated extracellular DNA relative to the NT. *p<0.05, **p<0.01, ***p<0.001 compared to NT (under media or DPI conditions) unless otherwise indicated. #p<0.05 compared to FM (under media or DPI conditions).