Neutrophils were exposed to no treatment (NT); LPS alone; unstimulated FM-CM (FM); or LPS-stimulated FM-CM (FM+LPS) in the presence of media or (A) the p38 MAPK inhibitor, SB203580 (n=11); (B) the ERK inhibitor, SCH772984 (n=9); or the JNK inhibitor, SP600125 (n=5). Barcharts show: (i) IL-8 secretion; (ii) MPO degranulation; and (iii) NET-associated extracellular DNA relative to the NT. *p<0.05, **p<0.01, ***p<0.001 compared to NT (under media or inhibitor conditions) unless otherwise indicated. #p<0.05 compared to FM (under media or inhibitor conditions).