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. 2019 Jun 20;39(6):BSR20190459. doi: 10.1042/BSR20190459

Figure 1. Effects of PHN alone in HEp-2 cells.

Figure 1

(A) Chemical structure of PHN. (B) Viability of HEp-2 cells treated with various concentrations of PHN for 12 h (orange), 24 h (green) and 48 h (purple) by CCK8 analysis. (C) HEp-2 cells were stained with FITC-Annexin V/PI and analyzed by flow cytometry, with different concentrations of PHN (0, 1, 10, 50 and 100 μM) treatment for 24 h. (D) Proteins associated with apoptosis were analyzed by Western blot. The expression level of cleaved-caspase 3, caspase 3, cleaved-caspase 9, caspase 9, β-actin and quantitation of cleaved-caspase 3/caspase 3 and cleaved-caspase 9/caspase 9 ratios. The experiment was repeated three times independently and data are expressed as mean ± standard error, *P<0.05. CCK-8, Cell counting Kit-8; PI, Propidium iodide.