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. 2019 Jul 3;10:441. doi: 10.3389/fendo.2019.00441

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Copyright © 2019 Huang, Zhao, Wang, Hillebrands, Born, Ji, An and Qin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological examination of non-diabetic adjacent normal kidney tissues (NC) and renal biopsy sections from DN patients. Representative images of (A) HE staining, (B) PAS staining, (C) Picrosirius red staining, and (D) Masson's trichrome staining for assessing TIF. Immunohistochemistry staining of (E) FN, (F) Col IV, and (G) STAT1 in human kidney sections. Original magnification, 100–200x. Quantitative analysis of (H) PAS staining, (I) Picrosirius red staining, (J) Masson's trichrome staining, (K) relative FN staining intensity, (L) relative Col IV staining intensity, and (M) relative STAT1 staining intensity. All data are expressed as means ± SEM, n = 10 (*P < 0.05 vs. NC).