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Effect of DAPA on renal production of fibrotic markers in diabetic mice. (A) Representative western blots for STAT1, TGF-β1, Col IV, and FN in renal cortical lysates. β-actin was used as internal control. (B) Density ratio of STAT1 to GAPDH mRNA expressions. (C–F) Normalized quantification of western blot data in diabetic mice at different times. *P < 0.05 vs. NC; **P < 0.05 vs. DM-8W; * &P < 0.05 vs. DM-12W; #P < 0.05 vs. DM-16W. Values are means ± SEM. n = 3.
