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. 2019 Jul 3;10:1526. doi: 10.3389/fmicb.2019.01526

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Copyright © 2019 Ruiz, Le Scornet, Sauviac, Rémy, Bruand and Meilhoc.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Organization of nirB, nirD, and narB genes on the S. meliloti genome. nirB, nirD, narB, and cysG genes are located on the S. meliloti megaplasmid pSymB as indicated at the top of the figure. The putative intergenic regions which are tested by PCR in the bottom part of the Figure are called (a) and (b). Total RNA isolated from cells grown in aerobic conditions in VMM without NH₄Cl in the presence of nitrate served as template for cDNA synthesis in the presence (cDNA) or in absence of reverse transcriptase enzyme (control, C). Genomic DNA was used as a positive control for PCR amplifications. The first lane (–) is a negative control without DNA.