Table 4.
Technical description of Perls' and Turnbull staining with brief description of correlation methodology with MRI.
| References | Technique | DAB | Slice thickness | Digitization | Correlation methodology |
|---|---|---|---|---|---|
| Chen et al., 1989 | Not described | No | / | No | Visual |
| Dhenain et al., 2002 | 30 min 2% PF 2% HCL | Yes | 7 μm | Yes | Visual |
| Fujii et al., 2007 | Not described | No | / | No | Visual |
| House et al., 2008 | 1 h 7% PF 3% HCL 37 °C | No | 6 μm | Yes | Visual |
| Tatsumi et al., 2008 | Not described | No | / | No | Visual |
| Matsusue et al., 2008 | Not described | No | / | No | Visual |
| van Rooden et al., 2009 | 15 h 7% PF 3% HCL | Yes | 5 μm | No | Visual |
| Schrag et al., 2010b | 20 min 10% PF 20% HCL | Yes | 8 μm | No | Visual |
| Fukunaga et al., 2010 | 2 h 5%PF 5% HCL 37 C | Yes | 20 μm | No | Visual |
| Bagnato et al., 2011 | 15 min 1% PF 1% HCL | Yes | 3-7 μm | Yes | Visual |
| De Reuck et al., 2011 | Not described | No | 12 μm | No | Visual |
| Massey et al., 2012 | Not described | No | 20 μm | Yes | Visual |
| Kwan et al., 2012 | 1 h 2% PF 2% HCL | Yes | 6 μm | No | Visual |
| Liem et al., 2012 | Not described | No | 10 μm | No | Visual |
| Blazejewska et al., 2013 | Not described | No | 5 μm | Yes | Visual after coregistration |
| Foroutan et al., 2013 | Time not given 10% PF 20% HCL | No | 5 μm | Yes | ROI to ROI |
| Walsh et al., 2013 | 30 min 1% PF 1% HCL | No | 8mm | Yes | ROI to ROI with optical density |
| Meadowcroft et al., 2015 | 30 min 2% PF 2% HCL | Yes | 60 μm | Yes | Visual |
| Stüber et al., 2014 | Not described | No | 30 μm | No | Visual |
| Sun et al., 2015 | 30 min 1% PF 1% HCL | No | 8mm | Yes | ROI to ROI with optical density |
| Wisnieff et al., 2015 | 1.5 h 5% PF 10% HCL then microwaved | No | 10 μm | Yes | Visual after coregistration |
| Lee et al., 2016 | Time not given 20% PF 20% HCL | No | 50 μm | Yes | Voxelwise correlation analyses after coregistration |
| van Veluw et al., 2016 | Not described | No | 6 μm | No | Visual |
| Wallace et al., 2016 | 2H 50% PF 2.5%HCL 37°C | Yes | 40 μm | Yes | Mean intensity in ROI after coregistration |
| De Reuck et al., 2017 | Not described | No | / | No | Visual |
| Massey et al., 2017 | Not described | No | 20 μm | No | Visual |
| Matsuda et al., 2017 | Not described | No | 6 μm | Yes | Visual after coregistration |
| Bulk et al., 2018b | 30 min 1% PF HCL for pH <5.5 | Yes | 20 μm | Yes | Pixel-based spatial correlation after coregistration |
| Kaunzner et al., 2019 | 30 min 4% PF 4% HCL | Yes | 5 μm | Yes | Visual |
| Lee et al., 2018 | Time not given 20% PF 20% HCL | No | 50 μm | Yes | Voxelwise correlation analyses after coregistration |
| Bagnato et al., 2011 | 90 min AS then 15 min 10% Pf 0.5% HCL | Yes | 3-7 μm | Yes | Visual |
| Dusek et al., 2016 | 90 min AS then 15 min 10% Pf 0.5% HCL | Yes | 5 μm | Yes | Area fraction in ROI after coregistration |
| Wallace et al., 2016 | 2 h 50% Pf 2.5% HCL 37 °C | Yes | 40 μm | Yes | Mean intensity in ROI after coregistration |
| Wiggermann et al., 2017 | 90 min AS then 15 min 10% Pf 0.5% HCL | Yes | 10 μm | Yes | Area fraction and mean intensity in ROI after coregistration |
| Bagnato et al., 2018 | 90 min AS then 15 min 10% Pf 0.5% HCL | Yes | 10 μm | Yes | Mean intensity in ROI after coregistration |
| Hametner et al., 2018 | 90 min AS then 15 min 10% Pf 0.5% HCL | Yes | 10 μm | Yes | Mean intensity in ROI without coregistration |
AS, Ammonium sulfide, HCL, hydrochloric acid; PF, potassium ferrocyanide; Pf, potassium ferricyanide; ROI, region of interest. when reaction temperature not given, assume room temperature. Solid line separates Perls' procedures (above) from Turnbull procedures (below).