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. 2019 Jul 3;7:118. doi: 10.3389/fcell.2019.00118

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Copyright © 2019 D’Souza, Blackburn, Kudlyk, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activity of the GARP complex is necessary for the formation and maintenance of EELSs. (A) HEK293T, VPS54 KO, and VPS54/COG4 DKO were created and analyzed for the presence of EELSs using phase-contrast microscopy. Scale bars are 50 μm. (B) Absence of knocked-out gene product was analyzed by Western Blot. (C) Airyscan images of WT, COG4 KO, VPS54 KO, and VPS54/COG4 DKO stained with the Golgi marker Giantin and late endo/lysosomal marker Lamp2. Lamp2 stained EELSs are only present in COG4 KO cells. Scale bars are 5 μm.