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. 2019 Jul 3;7:118. doi: 10.3389/fcell.2019.00118

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Copyright © 2019 D’Souza, Blackburn, Kudlyk, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EELSs are positive for late endocytic markers. HEK293T COG4 KO cells were transiently co-transfected with DNA constructs expressing Lamp2-mCherry and (A) GFP-CD63, (B) GFP-Rab7a, (C) GFP-Rab9a, (D) GFP-Rab39a, (E) GFP-STX8, and (F) GFP-Vamp7. Superresolution images were collected using Zeiss LSM880 AiryScan microscope. Graphs of intensity vs. distance represent colocalization between mCh and GFP. Scale bars are 10 μm.