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. 2019 Jul 3;7:118. doi: 10.3389/fcell.2019.00118

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Copyright © 2019 D’Souza, Blackburn, Kudlyk, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the EELSs by Electron microscopy. (A) Wild-type and (B–F) COG KO HEK293T cells grown on sapphire disks were high pressure frozen, fixed, embedded in Araldite 502/Embed 812 resin and analyzed by transmission electron microscopy. Note that the EELSs (labeled with star) are very prominent vacuole-like compartments in every COG KO cell.