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Activity of lysosomal enzyme Cathepsin B is absent in EELSs. HEK293T and COG4 KO cells were incubated with Magic Red substrate for Cathepsin B and Hoechst. Live cell images of DIC, DAPI and mCherry channels were acquired using Zeiss LSM880 microscope. Note the absence of Cathepsin B activity in large EELSs (arrows). Scale bars are 20 μm.
