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. 2019 Jul 3;7:118. doi: 10.3389/fcell.2019.00118

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Copyright © 2019 D’Souza, Blackburn, Kudlyk, Pokrovskaya and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biosynthetic cargo is delivered to EELSs with fast kinetic. (A) Schematic representation of the RUSH system experiment using reporter SBP-mCherry-Lamp1. (B) 30 min after release from ER mCherry-Lamp1 is localized in perinuclear Golgi region distinct from Lamp2-GFP-positive EELS. (C) After 120 min chase majority of mCherry-Lamp1 is seen within Lamp2-GFP EELSs. Superresolution images were collected using Zeiss LSM880 AiryScan microscope. Scale bars are 10 μm. Arrows indicate Lamp1 and Lamp2 positive EELSs.