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Experimental designs to measure cellular metabolic rates and nutrient solute diffusivities. (A) Schematic of porcine temporomandibular joint (TMJ) disc explant harvest cultured in a 24-well culture dish with 0.5, 1, 5, or 10 mM glucose media in a low-oxygen (2.5% or 5% oxygen level) incubator. (B) Schematic of TMJ disc explant harvest and custom solute diffusion chamber.
