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. 2019 Jul 3;9:554. doi: 10.3389/fonc.2019.00554

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Copyright © 2019 Belykh, Miller, Carotenuto, Patel, Cavallo, Martirosyan, Healey, Byvaltsev, Scheck, Lawton, Eschbacher, Nakaji and Preul.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation 2 confocal laser endomicroscope (CLE) images taken at different speeds. Comparison of CLE images taken with 3 various acquisition speed settings: (A) 135, (B,D) 1,080, and (C) 270. Images acquired at an intermediate speed setting (C) of 270 (calculated mean 2.5 frames per second) are of average quality and are able to resolve more structural details than images taken at the faster speed of 135 (A). Significant improvement in image quality at the slower speed of 1,080 (B,D) is mostly apparent in the areas with high contrast, such as small vessels when fluorescein sodium is used. When viewed as smaller pictures, the differences are less pronounced. However, on a large high-definition display, the image quality at different speeds differs substantially. Scale bars are 50 μm. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.