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. 2019 Jul 3;9:554. doi: 10.3389/fonc.2019.00554

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Copyright © 2019 Belykh, Miller, Carotenuto, Patel, Cavallo, Martirosyan, Healey, Byvaltsev, Scheck, Lawton, Eschbacher, Nakaji and Preul.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal laser endomicroscopy (CLE) patterns of GL261 glioma core. In vivo Gen2 CLE and rapid ex vivo laser scanning microscopy (LSM) images show higher fluorescein sodium (FNa) signal in the tumor area and visible contours of the tumor cells. Overall tissue architecture presents similarly in CLE and LSM images. (A) Most often tumor cells appeared darker than the background (arrows), while in some areas (B,C) tumor cells absorbed FNa and appeared bright. (D) Red blood cells, which were not stained by the Hoechst dye, are visible on a bright FNa background (yellow arrows). Scale bars are 50 μm. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.