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. 2019 Jul 3;9:554. doi: 10.3389/fonc.2019.00554

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Copyright © 2019 Belykh, Miller, Carotenuto, Patel, Cavallo, Martirosyan, Healey, Byvaltsev, Scheck, Lawton, Eschbacher, Nakaji and Preul.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tumor border 1 h after intravenous fluorescein sodium (FNa) injection. (A) Generation 2 (Gen2) and (C) generation 1 (Gen1) confocal laser endomicroscopy images show a similar cellular architecture pattern of the GL261 glioma border region. (B) Hematoxylin and eosin (H&E) image of the tumor border from a matched sample. (D) Larger field of view ex vivo benchtop confocal image shows gradient of fluorescein diffusion from the tumor to the normal brain. Arrows indicate invading tumor cell groups. LUT, look-up table. Scale bars are 50 μm. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.