FIGURE 1.

Schematic of (A) oxygen biosensor and (B) normothermic machine perfusion circuit. (A, I) Oxygen biosensor (3 × 0.5 mm) is implanted in liver tissue by injection through a 19 gauge needle. (II) Biosensor is excited by light from the optical reader. (III) Phosphorescence emitted by biosensor is proportional to tissue oxygen tension and detected by optical reader. (B) Schematic of the normothermic machine perfusion (NMP) circuit. NMP was performed using pressure-controlled flow through the portal vein. Acellular and cellular perfusates were tested.