Figure 1.

CD56^bright NK cells have unique tissue resident phenotype. Mononuclear cells isolated from liver perfusate (LP) and matched peripheral blood (PB) were stained with monoclonal antibodies. NK cells were identified from the CD45⁺ lymphocyte gate as CD56⁺CD3⁻. CD56^brightCD16^−/+ and CD56^dimCD16⁺ subsets were then gated and non-viable cells were excluded by FVS780 staining. (A) Representative histograms of Tbet, Eomes and CXCR6 expression in LP and PB NK cell subsets. (B) The MFI of Tbet in CD56^bright NK cells. (C) The MFI of Eomes in CD56^bright NK cells. (D) Percentage of CXCR6 positive CD56^bright NK cells. (E) The MFI of Tbet in CD56^dim NK cells. (F) The MFI of Eomes in CD56^dim NK cells. (G) Percentage of CXCR6 positive CD56^dim NK cells. Data presented as mean ± SEM (D,G) or box and whisker plots with minimum and maximum values (B,C,E,F). Data was analyzed using Wilcoxon matched pairs test (n = 10; *p < 0.05, **p < 0.01, and ***p < 0.001).