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. 2019 Jul 3;10:1502. doi: 10.3389/fimmu.2019.01502

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Copyright © 2019 Harmon, Jameson, Almuaili, Houlihan, Hoti, Geoghegan, Robinson and O'Farrelly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liver resident phenotype can be maintained by the addition of liver conditioned media. NK cells isolated from liver perfusate (LP) were cultured for 7 days, supplemented with 5 or 10% v/v liver conditioned media (LCM) and stained with monoclonal antibodies to assess transcription factor expression. (A) Representative histograms of Tbet, Eomes and CXCR6 expression from cells at day 0 (black line), day 7 untreated (blue line), day 7 5% LCM (orange line), and day 7 10% LCM (red line). (B) MFI of Tbet CD56^bright NK cells from LP at day 0 and 7 with LCM treatments. (C) MFI of Eomes in CD56^bright NK cells from LP at day 0 and 7 with LCM treatments. (D) Percentage of CXCR6 positive CD56^bright NK cells from LP at day 0 and 7 with LCM treatments. (E) MFI of Tbet in CD56^dim NK cells from LP at day 0 and 7 with LCM treatments. (F) MFI of Eomes in CD56^dim NK cells from LP at day 0 and 7 with LCM treatments. (G) Percentage of CXCR6 positive CD56^dim NK cells from LP at day 0 and 7 with LCM treatments. Data presented as mean ± SEM. Data was analyzed using Friedman test, with Dunn's multiple comparison test (n = 5; *p < 0.05).